Rabbit F(ab)2 anti-Mouse IgG (H+L)-unconj., MinX Hu, Polyclonal , Unconjugated

Product Overview

• Article Name: Rabbit F(ab)2 anti-Mouse IgG (H+L)-unconj., MinX Hu, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-710-4120
• Supplier Catalog Number: 710-4120
• Alternative Catalog Number: ROC-710-4120
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Conjugation: Unconjugated
• Alternative Names: Rabbit F(ab)2 Anti-Mouse IgG Antibody Pre-Adsorbed
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:10,000
• Application Notes: F(ab)2 Mouse IgG (H&L) Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated bindi

Images

SDS-PAGE of F(ab)2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Lane 1: Reduced F(ab)2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Lane 2: 5µL OPAL Pre-stained Marker MB-210-0500. Lane 3: Non-reduced F(ab)2 Rabbit anti-Mouse IgG Antibody min x Human serum proteins. Load: 1µg per lane. Predicted/Observed size: Non-reduced at 75 kDa , Reduced at 25 kDa.