Rabbit F(ab)2 anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal

Product Overview

• Article Name: Rabbit F(ab)2 anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal
• Biozol Catalog Number: ROC-710-4320
• Supplier Catalog Number: 710-4320
• Alternative Catalog Number: ROC-710-4320
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Rabbit F(ab)2 Anti-Mouse IgG Antibody peroxidase Conjugation, Rabbit Fab2 Anti-Mouse IgG HRP Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room

Images

ELISA Results of Rabbit F(ab)2 Anti-Mouse IgG Antibody Peroxidase Conjugated Min X human serum protein. Each well was coated in duplicate with 10 µg of Mouse IgG (p/n 010-0102). The working dilution is 87100. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066), TMB substrate (p/n TMBE-1000).