Rabbit F(ab)2 anti-Mouse IgG (H+L)-RPE, MinX Hu, Polyclonal

Product Overview

• Article Name: Rabbit F(ab)2 anti-Mouse IgG (H+L)-RPE, MinX Hu, Polyclonal
• Biozol Catalog Number: ROC-710-4820
• Supplier Catalog Number: 710-4820
• Alternative Catalog Number: ROC-710-4820
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: FACS,IF
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in rabbit.
• Conjugation: RPE
• Alternative Names: Rabbit F(ab)2 Anti-Mouse IgG Antibody phycoerythrin Conjugation, Rabbit Fab2 Anti-Mouse IgG PE Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
• Application Notes: F(ab)2 Mouse IgG Antibody Phycoerythrin Conjugated Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels,

Images

Dot Blot results of Rabbit F(ab)2 Anti-Mouse IgG Antibody Phycoerythrin Conjugated. Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit F(ab)2 Anti-Mouse IgG Antibody RPE at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.