0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
Application Notes:
F(ab)2 Anti-Rabbit IgG Phycoerythrin Conjugated Secondary Antibody has been tested by dot blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Dot Blot of Fab2 Goat anti-Rabbit IgG Antibody Phycoerythrin Conjugated. Antigen: Row 1: Rabbit IgG Row 2: Goat IgG Row 3: Mouse IgG Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng Primary antibody: N/A Secondary antibody: Fab2 Goat anti-Rabbit IgG Antibody Phycoerythrin Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 hour at RT
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