Goat F(ab)2 anti-Rabbit IgG (Fc)-Biotin, MinX none

Product Overview

• Article Name: Goat F(ab)2 anti-Rabbit IgG (Fc)-Biotin, MinX none
• Biozol Catalog Number: ROC-711-1603
• Supplier Catalog Number: 711-1603
• Alternative Catalog Number: ROC-711-1603
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG F(c) fragment
• Conjugation: Biotin
• Alternative Names: Goat F(ab)2 Anti-Rabbit IgG F(c) Biotin Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Fc Antibody Biotin Conjugation
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Rabbit IgG F(c) Biotin Antibody has been tested by western blot and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfoni

Images

Western Blot of Goat anti-F(ab)2 Rabbit IgG F(c) Antibody Biotin Conjugated Lane 1: Rabbit Fc Load: 100 ng per lane Primary antibody: F(ab)2 Rabbit IgG F(c) Antibody Biotin Conjugated at 1:1000 for overnight at 4C Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 28 kDa, 28 kDa