Goat F(ab)2 anti-Rabbit IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Rabbit IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-711-1622
• Supplier Catalog Number: 711-1622
• Alternative Catalog Number: ROC-711-1622
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Anti-Rabbit IgG F(ab)2 fragment was produced by repeated immunization with Rabbit IgG whole molecule in goat.
• Conjugation: Biotin
• Alternative Names: Goat F(ab)2 Anti-Rabbit IgG biotin conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody biotin Conjugation
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 anti-Rabbit IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detecti

Images

ELISA results of purified Goat F(ab)2 Anti-Rabbit IgG Antibody Biotin Conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins tested against purified Rabbit IgG (Purple Line). Each well was coated in duplicate with 1.0 µg of Rabbit IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).