Donkey F(ab)2 anti-Rabbit IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey F(ab)2 anti-Rabbit IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-711-701-127
• Supplier Catalog Number: 711-701-127
• Alternative Catalog Number: ROC-711-701-127
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey F(ab)2 Anti-Rabbit IgG Pre-Adsorbed Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Application Notes: F(ab)2 Anti-Rabbit IgG Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated bindi

Images

ELISA results of purified Donkey F(ab)2 Anti-Rabbit IgG Antibody Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins tested against purified Rabbit IgG. Each well was coated in duplicate with 1.0 µg of Rabbit IgG (p/n 011-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Goat anti-Donkey MX7 HRP conjugated, and TMB substrate (p/n TMBE-1000).