Goat F(ab)2 anti-Rat IgG (Fc)-FITC, MinX Bo,Ho,Hu, Polyclonal

Catalog Number: ROC-712-1222
Article Name: Goat F(ab)2 anti-Rat IgG (Fc)-FITC, MinX Bo,Ho,Hu, Polyclonal
Biozol Catalog Number: ROC-712-1222
Supplier Catalog Number: 712-1222
Alternative Catalog Number: ROC-712-1222
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: FLISA,FACS,IF
Species Reactivity: Rat
Immunogen: Rat IgG F(c) fragment
Conjugation: FITC
Alternative Names: Goat F(ab)2 Anti-Rat IgG F(c) Fluorescein Conjugated Antibody, Goat Fab2 Anti-Rat IgG Fc Antibody FITC Conjugation
Format: F(ab')2
Target Specificity: IgG (Fc)
Cross-Adsorption (MinX): Bovine,Equine,Human
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Rat
Antibody Type: Secondary Antibody
Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
Application Notes: F(ab)2 Anti-Rat IgG F(c) Fluorescein Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(
Dot Blot results of Goat F(ab)2 Anti-Rat IgG Antibody Fluorescein Conjugated. Dots are Rat F(c) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat F(ab)2 Anti-Rat IgG Antibody FITC at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, FITC filter.