Donkey Fab anti-Goat IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Donkey Fab anti-Goat IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-805-7602
• Supplier Catalog Number: 805-7602
• Alternative Catalog Number: ROC-805-7602
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Donkey Fab Anti-Goat IgG Biotin Conjugated Antibody, Donkey Fab Fragment Anti-Goat IgG Antibody Biotin Conjugation
• Format: Fab
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:600,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:20,000
• Application Notes: Fab Anti-Goat IgG Biotin Conjugated antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low

Images

ELISA results of purified Donkey Fab Anti-Goat IgG Antibody Biotin Conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).