Fab Anti-Mouse IgG (H&L) Rhodamine Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c)
Dot Blot results of Goat Fab Anti-Mouse IgG Antibody Rhodamine Conjugated. Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Mouse IgG TRITC at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.
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