Goat TrueBlot Set (IP Agarose beads), Clone: [eB270], Monoclonal
Biozol Catalog Number:
ROC-88-1488-31
Supplier Catalog Number:
88-1488-31
Alternative Catalog Number:
ROC-88-1488-31
Manufacturer:
Rockland Immunochemicals
Category:
Antikörper
Application:
IP, WB
Species Reactivity:
Goat
Conjugation:
HRP
Alternative Names:
Anti-Goat immunoglobulin Gamma, Agarose-conjugated IgG, Rb-a-Gt IgG, Rabbit-anti-Goat IgG, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, anti-Goat IgG HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting
Clonality:
Monoclonal
Clone Designation:
[eB270]
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Goat
Application Dilute:
IP: TrueBlot anti-Goat Ig IP Beads (binds 1 mg Ig/ml beads) have been reported for use in IP, WB: 1:1000
Application Notes:
Goat IgG TrueBlot is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enoug
Goat TrueBlot IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with goat anti-human Stat1 and immunoprecipitated using Protein G. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Goat TrueBlot ULTRA: Anti-Goat IgG HRP (lane 1) and conventional HRP-conjugated anti-goat polyclonal antibody (lane 2).
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