Rabbit TrueBlot Set (IP Agarose beads), Clone: [eB182], Monoclonal
Biozol Catalog Number:
ROC-88-1688-31
Supplier Catalog Number:
88-1688-31
Alternative Catalog Number:
ROC-88-1688-31
Manufacturer:
Rockland Immunochemicals
Category:
Antikörper
Application:
IP, WB
Species Reactivity:
Rabbit
Conjugation:
HRP
Alternative Names:
Anti-Rabbit Immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting
Clonality:
Monoclonal
Clone Designation:
[eB182]
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Rabbit
Application Dilute:
IP: TrueBlot anti-Rabbit Ig IP Beads (binds 2.5 mg Ig/mL beads) have been reported for use in IP, WB: 1:1000
Application Notes:
Rabbit IgG TrueBlot is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield eno
Rabbit TrueBlot IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP
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