Mouse TrueBlot Set (IP Agarose beads), Clone: [eB144], Monoclonal
Biozol Catalog Number:
ROC-88-7788-31
Supplier Catalog Number:
88-7788-31
Alternative Catalog Number:
ROC-88-7788-31
Manufacturer:
Rockland Immunochemicals
Category:
Antikörper
Application:
IP, WB
Species Reactivity:
Mouse
Conjugation:
HRP
Alternative Names:
Anti-Mouse immunoglobulin Gamma, Agarose-conjugated IgG, Anti-Ms IgG, Anti-Mouse IgG HRP, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting, Anti-Mouse IgG IP Agarose
Clonality:
Monoclonal
Clone Designation:
[eB144]
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Application Dilute:
IP: TrueBlot anti-Mouse Ig IP Beads (binds 0.4 mg Ig/ml beads) have been reported for use in IP, WB: 1:1000
Application Notes:
Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yiel
Mouse TrueBlot IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
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