Neurofilament L Antibody, IgG1, Clone: [DA2], Mouse, Monoclonal

Product Overview

• Article Name: Neurofilament L Antibody, IgG1, Clone: [DA2], Mouse, Monoclonal
• Biozol Catalog Number: ROC-900-301-D83
• Supplier Catalog Number: 900-301-D83
• Alternative Catalog Number: ROC-900-301-D83
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Bovine, Gallus, Human, Mouse, Rat
• Immunogen: Anti-NF-L Monoclonal Antibody was produced by repeated immunizations with preparation of swine intermediate filaments.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-Neurofilament L Antibody, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Product Properties

• Clonality: Monoclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• Clone Designation: [DA2]
• Isotype: IgG1
• NCBI: 4747
• UniProt: P02548
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Swine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IF Microscopy: 1:100, WB: 1:1000
• Application Notes: Anti-Neurofilament L antibody is tested for use in Western Blotting, ICC and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 68 kDa in size corresponding to the Neurofilament L proteins in Weste

Images

Western Blot of Mouse anti-Neurofilament L antibody. Lane 1: Rat cortex lysate. Lane 2: none. Load: 20 µg per lane. Primary antibody: Neurofilament L antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 68 kDa for Neurofilament L. Other band(s): none.