Titrated value sufficient to run approximately 10 mini blots.
Clone Designation:
[39C7]
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Yeast
Antibody Type:
Primary Antibody
Application Dilute:
IF Microscopy: 1:50-100
Application Notes:
Anti-Nuclear Pore Complex Antibody is tested for use in ICC/IF. Specific conditions for reactivity should be optimized by the end user
Immunofluorescence Microscopy of Mouse anti-Nuclear Pore Complex antibody. Tissue: HeLa cells. Fixation: 0.5% PFA Antigen retrieval: not required. Primary antibody: Nuclear Pore Complex antibody at 1:50-1:100 for 1 h at RT. Secondary antibody: Fluorescein mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Nuclear Pore Complex spans the double membrane of the nuclear membrane. Staining: Nuclear Pore Complex as green fluorescent signal with chicken anti-vimentin as red fluorescent signal.
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