Anti-Nuclei Antibody (Monoclonal) was produced by repeated immunizations with human cell homogenate.
Conjugation:
Unconjugated
Alternative Names:
mouse anti-Nuclei Antibody, HuNu, marker for human cells in xenographic research
Clonality:
Monoclonal
Concentration:
Titrated value sufficient to run approximately 10 mini blots.
Clone Designation:
[235-1]
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
IHC: 1:100, IF Microscopy: 1:100
Application Notes:
Anti-Nuclei (Mouse) antibody is tested for use in IHC and IF. Specific conditions for reactivity should be optimized by the end user. This antibody is directed against human nuclei.
Immunofluorescence Microscopy of Mouse anti-Nuclei antibody. Tissue: HeLa cells. Fixation: 0.5% PFA Antigen retrieval: not required. Primary antibody: Nuclei antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Nuclei is nuclear. Staining: Nuclei as green fluorescent signal.
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