Neurofilament M Antibody, IgG1, Clone: [3H11], Mouse, Monoclonal
Biozol Catalog Number:
ROC-912-301-D84
Supplier Catalog Number:
912-301-D84
Alternative Catalog Number:
ROC-912-301-D84
Manufacturer:
Rockland Immunochemicals
Host:
Mouse
Category:
Antikörper
Application:
IHC, WB
Species Reactivity:
Bovine, Gallus, Human, Mouse, Rat
Immunogen:
Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
Conjugation:
Unconjugated
Alternative Names:
mouse anti-Neurofilament M Antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Rat
Antibody Type:
Primary Antibody
Application Dilute:
IF Microscopy: 1:100, WB: 1:1000
Application Notes:
Anti-NFM antibody is tested for use in Western Blot, IHC, and IF. Expect a band of approximately 145 kDa in size corresponding to the NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications th
Western Blot of Mouse anti-Neurofilament M antibody. Lane 1: Rat cortex lysate. Lane 2: none. Load: 20 µg per lane. Primary antibody: Neurofilament M antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 145 kDa for Neurofilament M. Other band(s): none.
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