Biotin Peroxidase Conjugated

Catalog Number: ROC-B000-03
Article Name: Biotin Peroxidase Conjugated
Biozol Catalog Number: ROC-B000-03
Supplier Catalog Number: B000-03
Alternative Catalog Number: ROC-B000-03
Manufacturer: Rockland Immunochemicals
Category: Sonstiges
Application: DOT, ELISA
Conjugation: Biotin
Alternative Names: Vitamin H, Coenzyme R, Vitamin B7, Biotin Peroxidase Conjugated, Biotin HRP Conjugated
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Application Dilute: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
Application Notes: Biotin Peroxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assays target of interest is coupled with streptavidin.
Biotin Peroxidase Conjugated
Dot Blot of Biotin Peroxidase Conjugated. Antigen: Streptavidin. Load: Lane 1 - 100ng. Lane 2 - 33.3ng. Lane 3 - 11.1ng. Lane 4 - 3.70ng. Lane 5 - 1.23ng. Primary antibody: n/a. Secondary antibody: Biotin Peroxidase Conjugated at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.