Biotin Beta Galactosidase Conjugated

Product Overview

• Article Name: Biotin Beta Galactosidase Conjugated
• Biozol Catalog Number: ROC-B000-17
• Supplier Catalog Number: B000-17
• Alternative Catalog Number: ROC-B000-17
• Manufacturer: Rockland Immunochemicals
• Category: Sonstiges
• Application: DOT, ELISA
• Conjugation: Biotin
• Alternative Names: Vitamin H, Coenzyme R, Vitamin B7, beta-galactosidase, beta-gal, beta-gal, Biotin Beta Galactosidase Conjugated, Biotin Beta Gal Conjugated

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol, pH 7.8
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Biotin Beta Galactosidase Conjugated has been tested by ELISA and dot blot and is assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A wor

Images

BIOTIN BETA GALACTOSIDASE Conjugated

Dot Blot of BIOTIN Conjugated BETA GALACTOSIDASE. Lane 1: BIOTIN Conjugated BETA GALACTOSIDASE. Lane 2: BETA GALACTOSIDASE. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: None. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: 1% BSA-TTBS 30 min at RT.