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Brightfield WGA immunostaining in hippocampus of DBH-WGA (A,C) and wild type (B,D) mice.CA3 pyramidal cell bodies(A)were heavily filled with reaction product for WGA (white arrows inC). There was a clear demarcation at the CA3 border, with adjacent pyramidal neurons lacking reaction product (black arrows). Pyramidal neurons throughout the hippocampus of wild type mice lacked WGA reaction product. Scale bar is 500 µm inA,Band 100 µm inC,D. Preparation of the goat antiserum to WGA (Vector) consisted of preabsorption of the antibody (1:2000 in Tris B) with 2% mouse brain acetone powder (p/n C115) for 12-24 h at 4C. Figure 2. PMID: 22654744. |