Distribution of 5-ht7 receptor binding and mRNA in guinea-pig coronal forebrain sections dissected form guinea pig brains (p/n GP-T040). (a-d) Histological sections stained with 0.25% Cresyl violet. (e-h) Autoradiographs of total binding from same sections following incubation in 1.0 nm [3HJ-5-carboxamidotryptamine ([3H]-5-CT) in the presence of (-)-cyanopindolol and sumatriptan. (i-I) Autoradiographs from sections adjacent to those in h-n, with non-specific binding defined in the presence of 1µM 5-HT. (m-p) Autoradiographs following in situ hybridization using [35S]-UTP-labelled antisense (m, n, o) and sense (p) strand riboprobes. Darker areas correspond to higher mRNA levels. Section in panel m from a different experiment from n-p. Hybridization in ventromedial hypothalamus is dense just lateral to suprachiasmatic nuclei (arrowhead). Hybridization using sense strand probe was not above background except in hippocampal dentate gyrus (DG**). Abbreviations are defined in Table 2.Figure 5. PMID: 7647964.
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