AKT Isoform Antibody Sampler Kit, IgG2a, Clone: [various], Mouse, Monoclonal

Article Name:	AKT Isoform Antibody Sampler Kit, IgG2a, Clone: [various], Mouse, Monoclonal
Biozol Catalog Number:	ROC-K401
Supplier Catalog Number:	K401
Alternative Catalog Number:	ROC-K401
Manufacturer:	Rockland Immunochemicals
Host:	Mouse
Category:	Antikörper
Species Reactivity:	Human
Immunogen:	All Anti-AKT isoform specific Antibodies were produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1, AKT2 or AKT3 protein.
Conjugation:	Unconjugated
Alternative Names:	AKT isoform antibody kit, AKT-1, AKT-2, AKT-3, Protein kinase Akt antibody, Protein kinase B antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, antibody sampler,

Clonality:	Monoclonal
Concentration:	1.0 mg/ml by UV absorbance at 280 nm
Clone Designation:	[various]
Isotype:	IgG2a
NCBI:	31749
UniProt:	P31749
Buffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:	Liquid and Lyophilized
Target:	Human

Application Dilute:	ELISA: 1:2,000 - 1:10,000, Flow Cytometry: User Optimized, IHC: 20 µg/mL, WB: 1:1000
Application Notes:	Anti-AKT Antibodies are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT proteins by western blotting in the appropriate cell lysate or extract. All 3 primary monoclonal antib

	Plate was coated with monoclonal anti AKT1 antibody (capture antibody) followed by incubation with recombinant AKT1 (p/n 009-001-P21), AKT2 (p/n 009-001-P22), AKT3 (p/n 009-001-P23) proteins. Binding was detected with biotinylated monoclonal anti-AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3.

	Western Blot of Mouse Anti-AKT1 antibody. Lane 1: AKT-1 Null. Lane 2: WT. Lane 3: MEF 1. Lane 4 : A549 (p/n W09-001-GX4). Lane 5: Calu-1. Lane 6: PC-3 (p/n W09-001-GV6). Lane 7: HepG2 (p/n W09-001-GJ5). Lane 8: Jurkat (p/n W09-001-370). Lane 9: SKOV3 (p/n W09-001-GX9). Lane 10: HEK293T (p/n W09-001-GX5). Lane 11: C2C12 (p/n W10-001-GL7). Load: 20 ug per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 56 kDa for AKT1. Other band(s): none.
	AKT2 Antibody - Western Blot
	Western Blot of Mouse Anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
	Immunofluorescence of Mouse monoclonal anti-AKT3 antibody. Cell Type: A431 cells. Fixation: 4% paraformaldehyde 10 min. Permeabilization: 0.5% Triton X 30 min. Primary Ab: (p/n 200-301-J36) at 1:250 for 72 hours 4C. Secondary Ab: (p/n 610-142-121) at 1:1000 overnight 4C.
	Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4C.