1 Kit Components in this kit are sufficient to run 5 isotype class and subclass determinations.
Application Notes:
Each kit contains 5 packs of 2 cassettes each: one cassette for detecting IgG1, IgG2a and IgG2b, the other cassette for detecting IgG3, IgA and IgM. When a properly diluted sample containing a specific isotype antibody is added to the sample well, a spec
IsoMax(TM) Rapid Mouse Monoclonal Isotyping Kit results showing that for ascites fluid the darker red line indicates the class or subclass of antibody present. Often an additional weaker red line may appear indicating the presence of host (endogenous) serum immunoglobulins present in the ascites.
Rocklands IsoMax(TM) Isotyping Kit compares to an ELISA-format isotyping assay. Results demonstrate outstanding correlation between these two assay formats. The ELISA required controls, an ELISA plate and substrate and took 8 hours of hands-on time to coat the plates, block and subsequently perform the assay using 12 different antibodies. The IsoMax(TM) Isotyping Kit pipetting time was 10 minutes, all 20 assays were run simultaneously. Each cassette was read at 5 minutes. The total hands-on time for the IsoMax(TM) Kit, including scoring of the results, was under twenty minutes.
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