Intracellular ThiolEZ(TM) Cell Survival Assay Kit

Product Overview

• Article Name: Intracellular ThiolEZ(TM) Cell Survival Assay Kit
• Biozol Catalog Number: ROC-KLD-004
• Supplier Catalog Number: KLD-004
• Alternative Catalog Number: ROC-KLD-004
• Manufacturer: Rockland Immunochemicals
• Category: Kits/Assays
• Conjugation: Unconjugated
• Alternative Names: Total intracellular thiol Assay, glutathione and cysteine assay, cell viability, cell survival, cellular viability, cellular survival, antioxidant assay

Application Notes

• Application Notes: Intracellular ThiolEZ(TM) Cell Survival Assay Kit contains enough reagents for 100 assays using a 6-well plate.

Images

Pathways involved in thiol homeostasis in cells exposed to thiol modulators. Thiol modulators may react spontaneously with GSH and cysteine or in a reaction catalyzed by glutathione-S-transferase (GST) may produce disulfide of glutathione (GSH) or cysteine. Thiol modulators may also react with GSH to produce oxidized GSH (GSSG) and mixed disulfide with other molecules through the catalytic action of glutaredoxin (GRX). Thiol modulators may also enhance GSH either by bioreduction or synthesis.

Schematic representation of the various pathways involved in the cellular interactions of HEDS. HEDS reacts spontaneously with glutathione (GSH) or in a reaction catalyzed by glutathione-S-transferase (GST) to produce mixed disulfide (MESSG) of GSH and mercaptoethanol (ME) (Pathway 1). The mixed disulfide MESSG reacts with GSH and produces ME and GSSG by the catalytic action of glutaredoxin (GRX) (Pathway 2). The glutathione disulfide GSSG reacts with NADPH and produces GSH by the catalytic action of glutathione reductase (GRase) (Pathway 3). The conversion of GSSG to GSH i.e. GSH recycling requires NADPH recycling (NADP+ NADPH) by oxidative pentose phosphate cycle (OPPC) (Pathway 4)

Intracellular ThiolEZ(TM) Cell Survival Assay Kit is an easy to use assay for measurement of thiols in cells, it measures total intracellular thiols including glutathione and cysteine. Total ThiolEZ assay for intracellular total thiols with and without thiol enhancer and oxidant. y=0.301x+0.004 r2=0.989, y=0.597x-0.007 r2=0.999, y=0.222x-0.011 r2=0.988.