10X Phosphate Buffered Saline (PBS) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration which is 1X for this kit. SHEEP RED BLOOD CELLS (RBC) 10% Washed Poo
Sheep Hemagglutination Assay. Row A: Sheep Red Blood Cells 10% Washed Pooled Cells (p/n R405-0005) incubated in duplicate with control or antibody listed. Wells: (1-2) PBS only, (3-4) 0.8mg/mL Anti-RBC antibody (1:12.5 dilution), (5-6) 0.4mg/mL Anti-Sheep RBC antibody (1:25 dilution), (7-8) 0.2mg/mL Anti-Sheep RBC antibody (1:50 dilution), (9-10) 0.1mg/mL Anti-Sheep RBC antibody (1:100 dilution). Primary Antibody: Anti-Sheep Red Blood Cells (RABBIT) Antibody (p/n 213-4139-0002 Lot 40411) buffered with 1X PBS for 90 minutes at RT.
Sheep Hemagglutination Assay. Row A: Sheep Red Blood Cells 10% Washed Pooled Cells (p/n R405-0005) incubated in duplicate antibody listed. Wells: (1-2) 0.4mg/mL Anti-Sheep RBC antibody (1:25 dilution), (3-4) 0.2mg/mL Anti-Sheep RBC antibody (1:50 dilution). Primary Antibody: Anti-Sheep Red Blood Cells (RABBIT) Antibody (p/n 213-4139-0002 Lot 40411) buffered with 1X PBS for 90 minutes at RT.
(A) Red blood cells without agglutinating substances. The absence of these agglutinating substances will allow the red blood cells to pellet and form a dense button-like shape at the bottom of the well. (B) Red blood cells in the presence of agglutinating substances. In the presence of glycosylated proteins, lectins, or agglutinating viruses, the blood cells will clump around the agglutinating presence into a diffuse, suspended state.
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