ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
Biozol Catalog Number:
ROC-MB-064-1000
Supplier Catalog Number:
MB-064-1000
Alternative Catalog Number:
ROC-MB-064-1000
Manufacturer:
Rockland Immunochemicals
Category:
Kits/Assays
Application:
ELISA
Conjugation:
Unconjugated
Alternative Names:
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
Concentration:
1X
Buffer:
See application note.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized
Application Notes:
This product is a ready-to-use 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stab
Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.
ELISA results of ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) (p/n MB-064). Each well was coated in duplicate with 1.0 µg of Mouse IgG Whole Molecule. Blocking Buffer (p/n MB-064, lot35968) (Red), Blocking Buffer reference lot (Green), and Blocking Buffer control lot (Blue). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG, Goat anti-Rabbit HRP 1:8500, and TMB substrate.
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
* VAT and and shipping costs not included. Errors and price changes excepted
