Collagenase Type 3 is lower in secondary proteolytic contaminant activities but with typical collagenase activity. It is suggested for mammary primary cell isolation. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in balanced
The effect of CEC-sEVs/tPA on thrombosis and BBB leakage. CEC-sEVs/tPA significantly reduced embolus size at the origin of the occluded MCA (A), downstream FITC-plasma-perfused microvessels (B), Evans blue leakage (C), extravascular fibrin deposition assayed by double immunofluorescent staining (D, green), and intravascular platelet aggregation assayed by double immunofluorescent staining (E, green). The red color in panels D and E is EBA immunoreactive vessels. N=6/group, *p<0.05 and **p<0.01 vs Saline. Fig 2. PMID: 33557693.
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