1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Form:
Liquid
Application Dilute:
WB: User Optimized
Application Notes:
This 12 Epitope Tag Protein Marker Lysate has been tested in western blot and is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope ta
Western blot of 12-Epitope Tag Protein Marker lysate. The blot shows intense signal corresponding to the 12-Epitope Tag Protein Marker. The recommended load is 1 µl of lysate per lane for SDS-PAGE followed by western blotting. The membrane was probed with primary antibody at a 1:1,000 dilution in 5% BLOTTO in TBST overnight at 4C.
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