Protein A Biotin Conjugated

Product Overview

• Article Name: Protein A Biotin Conjugated
• Biozol Catalog Number: ROC-PA00-06
• Supplier Catalog Number: PA00-06
• Alternative Catalog Number: ROC-PA00-06
• Manufacturer: Rockland Immunochemicals
• Category: Proteine/Peptide
• Conjugation: Biotin
• Alternative Names: ProA, Staphylococcus A protein, Protein A Biotin Conjugated

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 38507
• UniProt: P38507
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
• Application Notes: Protein A Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.

Images

Signal response of Nobuto serum samples from 112 canids profiled with the F1-LPA. (A) Detection of F1 immunoreactivity was accomplished using a genus-specific secondary anti-IgG antibody. (B) Detection of F1 immunoreactivity was accomplished using biotinylated conjugates of staphylococcal protein A (p/n PA00-06), streptococcal protein G (p/n PG00-06), and staphylococcal protein A and streptococcal protein G (A/G), with sera diluted 1:20. The dashed line indicates the threshold for positive and negative samples using an S/N ratio of 10. FIG 2. PMID: 29695520.