Protein G is suitable for use as an antigen, as a control or standard in assays, for conjugation and for most other immunological methods. Protein G contains only IgG binding domains, ensuring maximum specific IgG binding.
Dot Blot of Protein G. Antigen 1: Human IgG (p/n 009-0102). Antigen 2: Rat IgG (012-0102). Antigen 3: Dog IgG (p/n 004-0102). Load: Starting dilution is 200 ng followed by a 3-fold serial dilution. Primary antibody: Protein G at 1:1000 for 60 min RT. Secondary antibody: Fluorescein rabbit anti-Protein G (p/n 200-402-138) at 1:1,000 for 30 min at RT. Block: MB-070 for 30 min at RT.
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