Levels of gamma-secretase components and active complexes in embryonic and adult rat brain.(A) The levels of gamma-secretase components in equal amounts of protein from embryonic (E) and adult (A) membrane preparations were analyzed by western blot using antibodies directed to the gamma-secretase complex components. (B) Quantification of gamma-secretase components in embryonic and adult membrane preparations from western blot. Equal amounts of protein from four different embryonic and adult membrane preparations were loaded together with three different dilutions of one of the membrane samples. The intensities from the diluted samples were plotted as a standard curve to which the other membrane preparations were correlated. Data are presented as mean values SD (n=4). *, p<0.05 adult vs embryo (C) Active gamma-secretase was captured from embryonic and adult rat brain membranes with 200 nM of a biotinylated gamma-secretase inhibitor in the presence or absence of 10 µM L-685,458 followed by the addition of streptavidin beads. Bound complexes and 10% of the input (IN) were separated on SDS-PAGE and detected by western blot using antibodies directed to the gamma-secretase components. Fig 3. PMID: 20333303.
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