TMB ELISA Peroxidase Substrate (BULK ORDER)

Product Overview

• Article Name: TMB ELISA Peroxidase Substrate (BULK ORDER)
• Biozol Catalog Number: ROC-TMBE-20L
• Supplier Catalog Number: TMBE-20L
• Alternative Catalog Number: ROC-TMBE-20L
• Manufacturer: Rockland Immunochemicals
• Category: Kits/Assays
• Application: ELISA
• Conjugation: Unconjugated
• Alternative Names: TMBE Peroxidase Substrate, TMB ELISA Peroxidase Substrate

Product Properties

• Concentration: 1X
• Form: Liquid - clear to very light blue colored liquid

Application Notes

• Application Dilute: ELISA: 1X
• Application Notes: TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes r

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TMB ELISA Peroxidase Substrate

Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.

The Rockland Immunochemical family of ELISA buffers includes coating buffers, blocking buffers, stabilization buffers, and substrates. Rockland TMBE ELISA Peroxidase Substrate is specially formulated for stability and sensitivity in HRP based ELISA systems.