Jurkat Whole Cell Lysate

Product Overview

• Article Name: Jurkat Whole Cell Lysate
• Biozol Catalog Number: ROC-W09-001-370
• Supplier Catalog Number: W09-001-370
• Alternative Catalog Number: ROC-W09-001-370
• Manufacturer: Rockland Immunochemicals
• Category: Zellen/Zellkultur
• Application: SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: Jurkat Whole Cell Lysate, Jurkat WCL, Jurkat Lysate

Product Properties

• Concentration: 1.0 mg/ml by BCA assay
• Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Source: Human
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: WB: User Optimized
• Application Notes: W09-001-370 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag

Images

Coomassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.