Human Foreskin Fibroblast Whole Cell Lysate, Human Foreskin Fibroblast WCL, HFF Cell Lysate, foreskin lysate
Concentration:
1.0 mg/mL by BCA assay
Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
W09-001-375 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Coomassie stained SDS-PAGE of 30 µg of Human Foreskin Fibroblast Whole Cell Lysate separated using a 4-20% gradient gel under reducing conditions (lane 2). Molecular weight standards are shown in lane 1.
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