HeLa Lysate MG-132 treated, Cell Lysate, MG-132 Stimulated Lysate, HeLa cells MG132 treated
Concentration:
1.0 mg/mL by BCA assay
Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Form:
Liquid
Application Dilute:
WB: User Optimized
Application Notes:
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been
SDS-PAGE results of HeLa Whole Cell Lysate - Trichostatin A Stimulated. Lane 1: HeLa Whole Cell Lysate - Trichostatin A Stimulated - reduced [10µg]. Lane 2: Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: HeLa Whole Cell Lysate - Trichostatin A Stimulated - non-reduced [10µg]. 4-20% gel. Coomassie stained.
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