1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
W09-001-MP5 has been tested by SDS-PAGE. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-m
SDS-PAGE Results of U-138 MG Whole Cell Lysate. Lane 1: U-138 MG Whole Cell Lysate (10µg) Reduced. Lane 2: Opal Prestained Molecular Weight Marker (p/n Mb-210-0500). Lane 3: U-138 MG Whole Cell Lysate (10µg) Non-Reduced. Results show wide range of molecular weight bands and no sign of degradation.
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