Human MAPK1 (ERK2) Knockout A549 Cell Lysate, Clone: [Clone 15], Monoclonal

Product Overview

• Article Name: Human MAPK1 (ERK2) Knockout A549 Cell Lysate, Clone: [Clone 15], Monoclonal
• Biozol Catalog Number: ROC-W09-K01-MU1
• Supplier Catalog Number: W09-K01-MU1
• Alternative Catalog Number: ROC-W09-K01-MU1
• Manufacturer: Rockland Immunochemicals
• Category: Antikörper
• Application: SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-ERK2 antibody, MAPK1, ERK 2, ERK-2, P42MAPK, PRKM1, PRKM2, MAPK 2, MAP kinase 2, Mitogen-activated protein kinase 2, p42-MAPK, MAP kinase isoform p42, Extracellular signal-regulated kinase 2, ERT1, MAP kinase 1, Mitogen-activated protein kinase 1

Product Properties

• Clonality: Monoclonal
• Concentration: 2.0 mg/mL by BCA assay
• Clone Designation: [Clone 15]
• NCBI: 28482
• UniProt: P28482
• Buffer: 1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors
• Source: Human
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, ChIP: User Optimized, IP: User Optimized, WB: User Optimized
• Application Notes: Human MAPK1 (ERK2) Knockout A549 Cell Lysate has been tested by SDS-PAGE and western blot and is suitable for use in Western blot, ELISA, Immunoprecipitation and ChIP. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when

Images

Western Blot of Human MAPK1 (ERK2) Knockout A549 Cell Lysate. Lane 1: Opal Prestained MW Marker (p/n MB-210-0500). Lane 2: A549 WCL Parental (p/n W09-001-372). Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental (p/n W09-000-364) Load: 10µg lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) (p/n 600-401-GP1) ~44kDa, [Blot B] stripped and re-probed with Anti-Tubulin (p/n 600-401-880) ~50kDa, at 1µg/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:30,000 for 1hr at RT. Block: BlockOut Buffer (p/n MB-073) for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.