NIH/3T3 Whole Cell Lysate

Product Overview

• Article Name: NIH/3T3 Whole Cell Lysate
• Biozol Catalog Number: ROC-W10-000-358
• Supplier Catalog Number: W10-000-358
• Alternative Catalog Number: ROC-W10-000-358
• Manufacturer: Rockland Immunochemicals
• Category: Zellen/Zellkultur
• Application: SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: NIH/3T3 Whole Cell Lysate, NIH/3T3 WCL, NIH/3T3 Lysate, NIH3T3 Lysate

Product Properties

• Concentration: 1.0 mg/ml by BCA assay
• Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Source: Mouse
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: WB: User Optimized
• Application Notes: W10-000-358 has been tested by SDS-PAGE and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag

Images

SDS-PAGE of NIH-3T3 Whole Cell Lysate. Lane 1: NIH-3T3 Whole Cell Lysate Reduced (10µg). Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: NIH-3T3 Whole Cell Lysate Non-Reduced (10µg). 4-20% Gel, Coomassie Stained.