1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Mouse
Form:
Liquid (sterile filtered)
Application Dilute:
WB: User Optimized
Application Notes:
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been
Coomassie stained SDS-PAGE of 20 µl of Mouse Embryonic Fibroblast (MEF) Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions. Arrows indicate the positions of molecular weight standards.
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