Total RNA was prepared from cell line WM1341D. WM1341D is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display epithelial square and triangular morphology in culture. This cell line features the specific V600R (Val600Arg) mutation at codon 600 in the BRAF gene. The V600R mutation results in an amino acid substitution at position 600 in BRAF, from a valine (V) to an arginine (R). This mutation occurs within the activation segment of the kinase domain. Mutations at V600 result in increased kinase activity and are transforming in vitro. WM1341D cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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