Total RNA was prepared from cell line WM1791C. WM1791C is a metastatic human melanoma cell line. This cell line features the wild type KRAS and KRAST501, KRASE62K mutation. This cell line also expresses a K22Q mutation in CDK4. The K22Q mutation results in an amino acid substitution at position 22 in CDK4, from a lysine (K) to a glutamine (Q). This cell line is wild type for BRAF, and c-KIT genes. WM1791C cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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