DNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
Source:
Human
Form:
Liquid
Application Notes:
Purified Genomic DNA is suitable for a number of molecular biology applications including but not limited to preparation of genomic libraries, PCR templates, DNA sequencing, DNA fingerprinting, and mutation analysis.
Human melanoma tumor cell genomic DNA isolated with genomic DNA miniprep kit
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