Total RNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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