DNA was prepared from cell line WM3061. WM3061 is a metastatic human melanoma cell line. These are small spherical cells that float easily after splitting and require 5% medium to adhere to culture flask. WM3061 cells produce xenograft tumors when injected into immunocompromised mice. This cell line contains a G13R mutation at position 13 in the N-RAS gene. Mutations affecting codon 13 lead to constitutive activation of RAS GTPase and ultimately neoplastic growth. The G13R is a rare NRAS mutation found in melanoma.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
Source:
Human
Form:
Liquid
Application Notes:
Purified Genomic DNA is suitable for a number of molecular biology applications including but not limited to preparation of genomic libraries, PCR templates, DNA sequencing, DNA fingerprinting, and mutation analysis.
Human melanoma tumor cell genomic DNA isolated with genomic DNA miniprep kit
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