Total RNA was prepared from cell line WM3061. WM3061 is a metastatic human melanoma cell line. These are small spherical cells that float easily after splitting and require 5% medium to adhere to culture flask. WM3061 cells produce xenograft tumors when injected into immunocompromised mice. This cell line contains a G13R mutation at position 13 in the N-RAS gene. Mutations affecting codon 13 lead to constitutive activation of RAS GTPase and ultimately neoplastic growth. The G13R is a rare NRAS mutation found in melanoma.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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