Total RNA was prepared from cell line WM3622. WM3622 is a metastatic human melanoma cell line that display large flat cell morphology. This cell line contains a F337S mutation in the PTEN gene. The codon for phe337 is replaced with a serine residue and this substitution could be highly detrimental to catalysis by PTEN and to its conformational stability. WM3622 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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