Total RNA was prepared from cell line WM3670. WM3670 is a metastatic human melanoma cell line with epithelial morphology. This cell line features the G469E mutation at codon 469 in the BRAF gene. The G469E mutation results in an amino acid substitution at position 469 in BRAF, from a glycine (G) to a glutamic acid (E). This mutation occurs within the highly conserved GXGXXG motif of the kinase domain. Most mutant BRAF proteins have increased kinase activity and are transforming in vitro. This cell line contains a G12D mutation at position 12 in the N-RAS gene. The G12D mutation results in an amino acid substitution at position 12 in N-RAS, from a glycine (G) to an aspartic acid (D).
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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