Total RNA was prepared from cell line WM3772. WM3772F is a primary human metastatic uveal melanoma cell line displaying clumpy pigmented morphology. These cells require 5% FBS to adhere to culture flask. This cell line was established from a metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. This cell line expresses wild type BRAF, N-RAS, c-KIT, and CDK4 genes. WM3772F cells match with WM3618F. These cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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