DNA was prepared from cell line WM3928. WM3928 is a human metastatic melanoma cell line that displays a mesenchymal morphology. This cell line was established from a left inguinal lymph node metastatic site of a patient. This cell line contains the PTEN Y177X substitution mutation. Y177 is a phosphorylation site in PTEN and mutation of Y177 leads to misregulation of PTEN function. This cell line contains wild type BRAF, N-RAS, c-KIT and CDK4 genes. WM3928 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
Source:
Human
Form:
Liquid
Application Notes:
Purified Genomic DNA is suitable for a number of molecular biology applications including but not limited to preparation of genomic libraries, PCR templates, DNA sequencing, DNA fingerprinting, and mutation analysis.
Human melanoma tumor cell genomic DNA isolated with genomic DNA miniprep kit
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