Non-viable cell pellet was generated from cell line WM852. WM852 is a metastaic melanoma cell line with epithelial morphology. This cell line was established from the metastatic site (abdomen) in a 63-year -old male. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. This cell line also expresses PTEN loss of function including hemizygous deletion. This cell line expresses wild type BRAF, c-KIT and CDK4 genes. WM852 cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
5.0 million cells (Count By Hemocytometer)
Buffer:
None
Source:
Human
Form:
Frozen Cell Pellet
Application Notes:
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Human melanoma tumor cell pellets for assessment of proteins and their phosphorylation status
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